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hek blue nfкb reporter cells  (InvivoGen)


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    InvivoGen hek blue nfкb reporter cells
    Hek Blue Nfкb Reporter Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hek+blue+nf%D0%BAb+reporter+cells/HEK-Blue+mTLR9+cells/pm36634584-100-0-7
    Average 95 stars, based on 69 article reviews
    hek blue nfкb reporter cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Construct:

    Article Title: Toll-like receptor 9 signaling after myocardial infarction: Role of p66ShcA adaptor protein.
    Article Snippet: .. Hek-Blue NFкB reporter cells overexpressing mouse TLR9 (Invivogen) were co-transfected (Lipofectamin 2000, Invitrogen) with pBabe construct containing cloned human p66ShcA gene or with empty pBabe vector. ..

    Clone Assay:

    Article Title: Toll-like receptor 9 signaling after myocardial infarction: Role of p66ShcA adaptor protein.
    Article Snippet: .. Hek-Blue NFкB reporter cells overexpressing mouse TLR9 (Invivogen) were co-transfected (Lipofectamin 2000, Invitrogen) with pBabe construct containing cloned human p66ShcA gene or with empty pBabe vector. ..

    Plasmid Preparation:

    Article Title: Toll-like receptor 9 signaling after myocardial infarction: Role of p66ShcA adaptor protein.
    Article Snippet: .. Hek-Blue NFкB reporter cells overexpressing mouse TLR9 (Invivogen) were co-transfected (Lipofectamin 2000, Invitrogen) with pBabe construct containing cloned human p66ShcA gene or with empty pBabe vector. ..



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    Colonization of epithelial cells. Adhesion of the toxigenic C. ulcerans strain KL756 and the non-toxigenic C. silvaticum isolate W25 to different human epithelial cell lines: ( a ) Detroit 562 cells, ( b ) HEK-Blue 293 hTLR2 cells, ( c ) HeLa cells. The respective cell line was seeded 24 h prior to infection and infected with bacteria at a multiplicity of infection (MOI) of 50 for 90 min. Columns and error bars represent the results and standard deviations of three independent biological replicates carried out with three technical replicates each ( n = 9). Adhesion efficiency was calculated based on the ratio of colony-forming units (CFU) prior to infection and CFU on the lysate plates after infection, multiplied by 100.

    Journal: International Journal of Molecular Sciences

    Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

    doi: 10.3390/ijms22073549

    Figure Lengend Snippet: Colonization of epithelial cells. Adhesion of the toxigenic C. ulcerans strain KL756 and the non-toxigenic C. silvaticum isolate W25 to different human epithelial cell lines: ( a ) Detroit 562 cells, ( b ) HEK-Blue 293 hTLR2 cells, ( c ) HeLa cells. The respective cell line was seeded 24 h prior to infection and infected with bacteria at a multiplicity of infection (MOI) of 50 for 90 min. Columns and error bars represent the results and standard deviations of three independent biological replicates carried out with three technical replicates each ( n = 9). Adhesion efficiency was calculated based on the ratio of colony-forming units (CFU) prior to infection and CFU on the lysate plates after infection, multiplied by 100.

    Article Snippet: HEK-Blue 293 hTLR2 , Human TLR2/NFкB/SEAP reporter HEK293 cells , Invivogen.

    Techniques: Infection

    TLR2 receptor activation by C. glutamicum ATCC13032, C. ulcerans KL756 and C. silvaticum W25 . HEK-Blue 293 hTLR2 receptor cells were infected at a MOI of 100 (white), 10 (light grey) and 1 (dark grey) with viable (*) and UV-killed (†) bacteria. Heat-killed Listeria monocytogenes (HKLM) (blue) were used as positive and phosphate-buffered saline (PBS) (light blue) as negative control. Experiments were performed in three independent biological replicates each in technical triplicates and mean values and standard deviations are shown.

    Journal: International Journal of Molecular Sciences

    Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

    doi: 10.3390/ijms22073549

    Figure Lengend Snippet: TLR2 receptor activation by C. glutamicum ATCC13032, C. ulcerans KL756 and C. silvaticum W25 . HEK-Blue 293 hTLR2 receptor cells were infected at a MOI of 100 (white), 10 (light grey) and 1 (dark grey) with viable (*) and UV-killed (†) bacteria. Heat-killed Listeria monocytogenes (HKLM) (blue) were used as positive and phosphate-buffered saline (PBS) (light blue) as negative control. Experiments were performed in three independent biological replicates each in technical triplicates and mean values and standard deviations are shown.

    Article Snippet: HEK-Blue 293 hTLR2 , Human TLR2/NFкB/SEAP reporter HEK293 cells , Invivogen.

    Techniques: Activation Assay, Infection, Negative Control

    HEK 293 hTLR2 reporter cell assay. Cells were infected with viable (*) and UV-killed (†) bacteria at MOI 100, MOI 10 and MOI 1 for 16 h. Heat-killed L. monocytogenes (HKLM) served as positive and PBS as negative control.

    Journal: International Journal of Molecular Sciences

    Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

    doi: 10.3390/ijms22073549

    Figure Lengend Snippet: HEK 293 hTLR2 reporter cell assay. Cells were infected with viable (*) and UV-killed (†) bacteria at MOI 100, MOI 10 and MOI 1 for 16 h. Heat-killed L. monocytogenes (HKLM) served as positive and PBS as negative control.

    Article Snippet: HEK-Blue 293 hTLR2 , Human TLR2/NFкB/SEAP reporter HEK293 cells , Invivogen.

    Techniques: Infection, Negative Control

    Bacterial strains, plasmids and cell lines used in this study.

    Journal: International Journal of Molecular Sciences

    Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

    doi: 10.3390/ijms22073549

    Figure Lengend Snippet: Bacterial strains, plasmids and cell lines used in this study.

    Article Snippet: HEK-Blue 293 hTLR2 , Human TLR2/NFкB/SEAP reporter HEK293 cells , Invivogen.

    Techniques:

    Colonization of epithelial cells. Adhesion of the toxigenic C. ulcerans strain KL756 and the non-toxigenic C. silvaticum isolate W25 to different human epithelial cell lines: ( a ) Detroit 562 cells, ( b ) HEK-Blue 293 hTLR2 cells, ( c ) HeLa cells. The respective cell line was seeded 24 h prior to infection and infected with bacteria at a multiplicity of infection (MOI) of 50 for 90 min. Columns and error bars represent the results and standard deviations of three independent biological replicates carried out with three technical replicates each ( n = 9). Adhesion efficiency was calculated based on the ratio of colony-forming units (CFU) prior to infection and CFU on the lysate plates after infection, multiplied by 100.

    Journal: International Journal of Molecular Sciences

    Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

    doi: 10.3390/ijms22073549

    Figure Lengend Snippet: Colonization of epithelial cells. Adhesion of the toxigenic C. ulcerans strain KL756 and the non-toxigenic C. silvaticum isolate W25 to different human epithelial cell lines: ( a ) Detroit 562 cells, ( b ) HEK-Blue 293 hTLR2 cells, ( c ) HeLa cells. The respective cell line was seeded 24 h prior to infection and infected with bacteria at a multiplicity of infection (MOI) of 50 for 90 min. Columns and error bars represent the results and standard deviations of three independent biological replicates carried out with three technical replicates each ( n = 9). Adhesion efficiency was calculated based on the ratio of colony-forming units (CFU) prior to infection and CFU on the lysate plates after infection, multiplied by 100.

    Article Snippet: HEK-Blue 293 hTLR9 , Human TLR9/NFкB/SEAP reporter HEK293 cells , Invivogen.

    Techniques: Infection

    TLR2 receptor activation by C. glutamicum ATCC13032, C. ulcerans KL756 and C. silvaticum W25 . HEK-Blue 293 hTLR2 receptor cells were infected at a MOI of 100 (white), 10 (light grey) and 1 (dark grey) with viable (*) and UV-killed (†) bacteria. Heat-killed Listeria monocytogenes (HKLM) (blue) were used as positive and phosphate-buffered saline (PBS) (light blue) as negative control. Experiments were performed in three independent biological replicates each in technical triplicates and mean values and standard deviations are shown.

    Journal: International Journal of Molecular Sciences

    Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

    doi: 10.3390/ijms22073549

    Figure Lengend Snippet: TLR2 receptor activation by C. glutamicum ATCC13032, C. ulcerans KL756 and C. silvaticum W25 . HEK-Blue 293 hTLR2 receptor cells were infected at a MOI of 100 (white), 10 (light grey) and 1 (dark grey) with viable (*) and UV-killed (†) bacteria. Heat-killed Listeria monocytogenes (HKLM) (blue) were used as positive and phosphate-buffered saline (PBS) (light blue) as negative control. Experiments were performed in three independent biological replicates each in technical triplicates and mean values and standard deviations are shown.

    Article Snippet: HEK-Blue 293 hTLR9 , Human TLR9/NFкB/SEAP reporter HEK293 cells , Invivogen.

    Techniques: Activation Assay, Infection, Negative Control

    HEK 293 hTLR2 reporter cell assay. Cells were infected with viable (*) and UV-killed (†) bacteria at MOI 100, MOI 10 and MOI 1 for 16 h. Heat-killed L. monocytogenes (HKLM) served as positive and PBS as negative control.

    Journal: International Journal of Molecular Sciences

    Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

    doi: 10.3390/ijms22073549

    Figure Lengend Snippet: HEK 293 hTLR2 reporter cell assay. Cells were infected with viable (*) and UV-killed (†) bacteria at MOI 100, MOI 10 and MOI 1 for 16 h. Heat-killed L. monocytogenes (HKLM) served as positive and PBS as negative control.

    Article Snippet: HEK-Blue 293 hTLR9 , Human TLR9/NFкB/SEAP reporter HEK293 cells , Invivogen.

    Techniques: Infection, Negative Control

    Bacterial strains, plasmids and cell lines used in this study.

    Journal: International Journal of Molecular Sciences

    Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

    doi: 10.3390/ijms22073549

    Figure Lengend Snippet: Bacterial strains, plasmids and cell lines used in this study.

    Article Snippet: HEK-Blue 293 hTLR9 , Human TLR9/NFкB/SEAP reporter HEK293 cells , Invivogen.

    Techniques: